Description: Segment 1 Opening credits. Prof. Greaves introduces the topic of inflammatory reaction of skin. Behind him is a picture of a woman's dermatitis-inflamed breasts. He describes other stimuli that provoke inflammatory reactions, including allergic reactions and physical injury. He explains what an inflammatory reaction is, using animated graphs, saying that much work has been put into finding calming measures for inflamed skin but not enough work has been put into the mechanisms of the reaction. He discusses mediators of inflammation such as histamine and prostaglandins. He discusses prostaglandins in more detail. Time start: 00:00:00:00 Time end: 00:05:42:15 Length: 00:05:42:15 Segment 2 Greaves introduces Dr Kingston, who discusses some aspects of the biochemistry of prostaglandins and their mode of enzymic synthesis in tissues. He uses diagrams and slides throughout his talk. First he describes the basic structure of prostaglandins, then their synthesis. Greaves and Kingston appear together. A diagram of prostaglandin E is seen. Greaves explains that Kingston has been injected with 1 microgram of prostaglandin E1. A close-up of Kingston's arm is seen, which is slightly inflamed around the site of injection. He touches the area to show that this touch causes Kingston pain. Time start: 00:05:42:15 Time end: 00:10:26:13 Length: 00:04:43:23 Segment 3 Greaves talks about adding a subsequent pain-producing agent such as bradykinin. He then talks about demonstrating the presence of increased concentrations of prostaglandin E1 in inflamed skin. He introduces another experiment in this experiment, suction blisters have been raised on the skin of a female. Footage of the female is seen with suction blisters on her belly. The contents of the blisters are aspirated using a needle and syringe and then tested for prostaglandin activity. He talks about the cascade superfusion technique used to examine the contents of the suction blisters. Film of the technique and the apparatus needed is seen. He explains the result of the procedure and talks further about this experiment. Time start: 00:10:26:13 Time end: 00:15:21:00 Length: 00:04:54:12 Segment 4 Next, Dr Kingston talks about the relationship between non-steroid anti-inflammatory drugs and prostaglandin activity. Still and animated images are seen throughout his talk. A graph shows the relationship between the inhibition of prostaglandin synthesis and aspirin dosage. Greaves ends the lecture by summarising the relationship between prostaglandin activity and inflammatory reaction. Time start: 00:15:21:00 Time end: 00:20:23:15 Length: 00:05:02:15